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. 2012 Aug 10;13(10):923–929. doi: 10.1038/embor.2012.119

Figure 4.

Figure 4

C. elegans thoc-2 mutants show meiosis failure and increased levels of SPO-11-independent RAD-51 foci. (A) Representative images of diplotene-diakinesis germline region of fixed germlines counterstained with DAPI. (B) Representative images of fixed germlines immunostained with anti-RAD-51 antibodies and counterstained with DAPI, and quantification of RAD-51 foci of germline nuclei. Zones 1–2 (mitosis), zone 3 (transition zone), zones 4–5–6 (pachytene). The number of nuclei was scored in 1-day post-L4 stage animals. At least 10 germlines per strain were scored for each experiment (n=30). Scale bar, 10 μm. DAPI, 4,6-Diamidino-2-phenylindole; NA, not applicable; WT, wild-type.