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. 2012 Aug 10;13(10):923–929. doi: 10.1038/embor.2012.119

Figure 5.

Figure 5

Replication is impaired in C. elegans thoc-2 germlines and partially alleviated by RNase H microinjection. (A) Quantification of Cy3-dUTP incorporation 24 and 48 h after microinjection in the germline regions of mitosis (MT), transition zone (TZ), early-, mid- and late-pachytene (PQ) and diplotene (DT). Error bars indicate standard error of mean from at least 10 germlines for each experiment (n=30). (B) Representative images of fixed germlines of the indicated genotype of adult hermaphrodites 16 h after microinjection with Cy3-dUTP and with or without RNase H and quantification of Cy3-dUTP incorporation. Error bars indicate standard error of mean from at least 10 germlines for each experiment (n=15). Statistically significant differences with respect to N2(wt) (P<0.001) are indicated by an asterisk ‘*’ (Student’s t-test). Scale bar, 5 μm. DAPI, 4,6-Diamidino-2-phenylindole; WT, wild-type.