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. Author manuscript; available in PMC: 2012 Oct 4.
Published in final edited form as: J Biol Chem. 2005 Oct 31;281(2):1119–1127. doi: 10.1074/jbc.M509421200

FIGURE 4. Effects of T127L/S128I, T127L/S128A, and WT CRP proteins on the growth of host E. coli strains.

FIGURE 4

Overnight cultures of the UQ3588 host strain (Table 1) expressing different CRP proteins (C-terminally His-tagged) under IPTG-dependent regulation were diluted in LC medium (see “Experimental Procedures”) supplemented with 50 μg/ml ampicillin, 0–1 mM IPTG, and 0 cAMP (A–C) or 2 mM cAMP (D–F). Open symbols, no IPTG; lightgraysymbols,0.01mM IPTG;darkgraysymbols,0.1mM IPTG;blacksymbols,1mM IPTG. Each strain was grown with shaking at 37 °C and 220 rpm and sampled every hour for the measurement of A600 nm.