TABLE 1.
Bacterial strains, plasmids, and promoters used in this work
| Brief description | Source/Ref. | |
|---|---|---|
| Bacterial strains | ||
| HB101 | ||
| M182 | Δ(lacIPOZY) × 74galK galU strA | Ref. 46 |
| UQ3259 | F− his StrR relA1 ilv∷Tn10 cya (SA2755) | S. Adhya |
| UQ3260 | F− his StrR relA1 crp∷cam (SA2777) | S. Adhya |
| UQ3261 | F− his StrR relA1 ilv∷Tn10 cya and crp∷cam | This work |
| UQ3588 | HB101 with ilv∷Tn10 cya and crp∷cam recA56 TnR | This work |
| UQ3740 | M182 carrying λ prophage with CC(−41.5)∷lacZ fusion (RLG4649) | Ref. 26 |
| UQ3741 | M182 carrying λ prophage with CC(−61.5)∷lacZ fusion (RLG4650) | Ref. 27 |
| UQ3809 | UQ3740 with ilv∷Tn10 cya and crp∷cam | This work |
| UQ3811 | UQ3741 with ilv∷Tn10 cya and crp∷cam | This work |
|
| ||
| Plasmids | ||
| pEXT20 | Ref. 32 | |
| pHY26-1 | pEXT20 plasmid bearing E. coli crp allele (His6-tagged) | This work |
|
| ||
| Promoters | ||
| pSR | pBR322 derivative containing λ OOP transcription terminator | Ref. 47 |
| pSR/CC(−41.5) | Class II derivative of melR promoter with consensus CRP-binding site centered at −41.5 | Ref. 26 |
| pSR/CC(−61.5) | Class I derivative of melR promoter with consensus CRP-binding site centered at −61.5 | Ref. 27 |