Bacterial strains
|
|
|
HB101 |
|
|
M182 |
Δ(lacIPOZY) × 74galK galU strA
|
Ref. 46
|
UQ3259 |
F− his StrR relA1 ilv∷Tn10 cya (SA2755) |
S. Adhya |
UQ3260 |
F− his StrR relA1 crp∷cam (SA2777) |
S. Adhya |
UQ3261 |
F− his StrR relA1 ilv∷Tn10 cya and crp∷cam
|
This work |
UQ3588 |
HB101 with ilv∷Tn10 cya and crp∷cam recA56 TnR
|
This work |
UQ3740 |
M182 carrying λ prophage with CC(−41.5)∷lacZ fusion (RLG4649) |
Ref. 26
|
UQ3741 |
M182 carrying λ prophage with CC(−61.5)∷lacZ fusion (RLG4650) |
Ref. 27
|
UQ3809 |
UQ3740 with ilv∷Tn10 cya and crp∷cam
|
This work |
UQ3811 |
UQ3741 with ilv∷Tn10 cya and crp∷cam
|
This work |
|
Plasmids
|
|
|
pEXT20 |
|
Ref. 32
|
pHY26-1 |
pEXT20 plasmid bearing E. coli crp allele (His6-tagged) |
This work |
|
Promoters
|
|
|
pSR |
pBR322 derivative containing λ OOP transcription terminator |
Ref. 47
|
pSR/CC(−41.5) |
Class II derivative of melR promoter with consensus CRP-binding site centered at −41.5 |
Ref. 26
|
pSR/CC(−61.5) |
Class I derivative of melR promoter with consensus CRP-binding site centered at −61.5 |
Ref. 27
|