TABLE 2. Screening results for constitutively active CRP variants randomly altered at Th127 and Ser128.
Screened without IPTG |
Screened with 100 μM IPTG |
|||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CRP | Position 127 |
Position 128 |
CRP | Position 127 |
Position 128 |
CRP | Position 127 |
Position 128 |
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Codon | Amino acid | Codon | Amino acid | Codon | Amino acid | Codon | Amino acid | Codon | Amino acid | Codon | Amino acid | |||
LB-1a | TTA | Leu | ATA | Ile | DB-1'b | ATA | Ile | ATT | Ile | LB-1'c | ATG | Met | ACA | Thr |
LB-2 | CTG | Leu | ATT | Ile | DB-2' | ATG | Met | GTA | Val | LB-2' | TTG | Leu | CAA | Gln |
LB-3 | CTG | Leu | ATA | Ile | DB-3' | CTT | Leu | GTG | Val | LB-3' | TTG | Leu | CTA | Leu |
LB-4 | CTG | Leu | ATT | Ile | DB-4' | TTG | Leu | GTC | Val | LB-4' | ACA | Thr | ATA | Ile |
LB-5 | TTG | Leu | ATC | Ile | DB-5' | CTA | Leu | GTG | Val | LB-5' | CTG | Leu | ACT | Thr |
LB-6 | CTG | Leu | ATA | Ile | DB-6' | CTT | Leu | GTG | Val | LB-6' | CTC | Leu | ATG | Met |
LB-7 | TTA | Leu | ATT | Ile | DB-7' | ATG | Met | GTA | Val | LB-7' | CTA | Leu | ACT | Thr |
LB-8 | TTA | Leu | ATA | Ile | DB-8' | CTA | Leu | AAG | Lys | LB-8' | ATG | Met | ATG | Met |
Light blue (2.3% of the total population).
Dark blue (3.4% of the total population).
Light blue (8.5% of the total population).