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. 2012 Oct;343(1):82–90. doi: 10.1124/jpet.112.192286

Fig. 1.

Fig. 1.

Effects of spermine and ifenprodil at GluN1/GluN2B receptors containing GluN1 and GluN2B mutants. The effects of spermine (100 μM) and ifenprodil (1 μM) were determined in oocytes expressing GluN1/GluN2B receptors with wild-type (WT) and mutant GluN1 or GluN2B subunits, voltage-clamped at −20 mV and activated by 10 μM glutamate and 10 μM glycine. Values are mean ± S.E.M. from 20 oocytes for wild-type and four oocytes for each mutant. A, mutations that show stimulation <115% (approximately 70% decrease in spermine stimulation compared with wild type) in the presence of spermine are highlighted by open columns. The solid line (100%) indicates the activity in the absence of spermine, and the dotted line indicates the activity in the presence of spermine in wild-type GluN1/GluN2B receptors. B, mutations in which currents are >80% of control in the presence of ifenprodil (approximately 70% decrease in ifenprodil inhibition compared with the wild type) are highlighted by open columns. The solid line (100%) indicates the activity in the absence of ifenprodil, and the dotted line indicates the activity in the presence of ifenprodil in wild-type GluN1/GluN2B receptors. Mutants shown with black letters were identified previously, and mutants shown with white letters in a black box were newly identified.