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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Free Radic Biol Med. 2012 Mar 1;52(9):1806–1819. doi: 10.1016/j.freeradbiomed.2012.02.029

Figure 2. Nox5-derived superoxide and hydrogen peroxide are reduced by NO.

Figure 2

(A) 48hr after transfection in COS-7 cells, SOD(100U/ml) or Tiron(1mM) were added to the cells. Superoxide was measured by chemiluminescence and expressed as % of control. (B) Superoxide release in COS-7 cells expressing RFP, Nox5 or iNOS in the presence and absence of L-NAME (1mM) (C) COS-7 cells expressing Nox5 were treated with or without DETA NONOate (1mM), and superoxide was measured by cytochrome c reduction. Data is expressed as nmol/10^6 cells/min. (D) Superoxide was measured in COS-7 cells expressing Nox5 with or without iNOS using lucigenin-chemiluminescence. (E) H2O2 was measured by Amplex Red 48hrs after co-transfection. Data is expressed as % of control (means ± S.E., n =6 * p<0.05 versus Nox5+RFP).