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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Free Radic Biol Med. 2012 Mar 1;52(9):1806–1819. doi: 10.1016/j.freeradbiomed.2012.02.029

Figure 4. Inhibition of Nox5 can be reversed by persistent, but not acute inhibition of NO production.

Figure 4

(A) COS-7 cell were co-transfected with Nox5 and either iNOS or RFP (control). 48h after transfection, cells were treated with L-NAME (1mM) for 1h and superoxide was measured (B) L-NAME (100μM) was added for 24h following transfection. Data is expressed as % of control (means ± S.E., n =6 * p<0.05 versus Nox5+RFP, # p<0.05 versus Nox5+iNOS). (C) After 48hr transfection, cells were treated with L-NAME(1mM), NO release was measured by chemiluminescence (means ± S.E., n =6 * p<0.05 versus no L-NAME).