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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Free Radic Biol Med. 2012 Mar 1;52(9):1806–1819. doi: 10.1016/j.freeradbiomed.2012.02.029

Figure 8. NO-inhibition of Nox5 is reversible and is prevented by the denitrosylases Trx1 and GSNOR.

Figure 8

(A) COS-7 cells expressing Nox5 were exposed to 1mM DETA NONOate and superoxide production was monitored over time. (B-C) COS-7 cells were co-transfected with Nox5, RFP or iNOS with or without the denitrosylases, thioredoxin1 (Trx1) or GSNO reductase (GSNOR). Superoxide production is expressed as % of control (means ± S.E., n =6 * p<0.05). (D) Measurement of NO release in cells expressing iNOS with or without Trx1 and GSNOR. (E) Superoxide release from HEK cells stably expressing Nox5 with or without either iNOS, Trx1 or GSNOR. (F) HEK cells stably expressing Nox5 were co-transfected with iNOS and either control (RFP), Trx1 or GSNOR. Data are presented as means ± SE (n = 4-6 * p<0.05).