FIG. 2.
Validation in human ESCs and iPS cells. (A) RT-PCR of pluripotency genes in 2 hESC lines, hiPS cells and partially reprogrammed iPS cells (#1 and #2). (B) Photos of hESCs, hiPS, and partially reprogrammed iPS clones revealing morphological differences with the pluripotent state. (C) Validation by the western blot methodology of cyclin A1 and cdc27 in hESCs, hiPS clones and partially reprogrammed hiPS clones. Note that with reduced pluripotent potential in the partially reprogrammed iPS cells (#1 and #2), cyclin A1 protein levels increase compared to hESCs. hESCs have low levels of cyclin A1 protein expression and higher pluripotent potential. Color images available online at www.liebertonline.com/scd