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. 2012 Oct;32(10):474–484. doi: 10.1089/jir.2012.0031

FIG. 7.

FIG. 7.

Effect of NF-κB p65-induced knockdown by siRNA transfection in vivo on IL-1β-induced increase in mouse intestinal MLCK expression. (A) NF-κB p65 siRNA transfection in vivo prevented the IL-1β-induced increase in the mouse intestinal MLCK mRNA transcript as assessed by real-time PCR. *P<0.001 versus control. (B) NF-κB p65 siRNA transfection in vivo prevented the IL-1β-induced increase in mouse intestinal MLCK protein expression as assessed by Western blot analysis. (C) NF-κB p65 siRNA transfection in vivo prevented the IL-1β-induced increase in mouse intestinal mucosal-to-serosal flux of dextran 10KD. *P<0.001 versus siRNA nontarget; **P<0.001 versus siRNA NF-κB p65.