FIG. 2.

Generation of the MiPSCs from hAMSCs. (A) Timeline of the MiPSC generation from the hAMSCs demonstrates the first ESC-like colony found within the first 11 days after transduction. At day 20, the characteristic iPSC colonies were observed, which displayed a cobblestone appearance with prominent nucleoli and a distinct individual cell border. (B) The MiPSCs demonstrated high alkaline phosphatases activity similar to the H7 hESCs but that was not found in the hAMSCs. (C) Teratoma derived from the MiPSCs after transplantation into the hind limbs of SCID mouse was found 5 weeks after the injection. Hematoxylin and eosin stain confirmed that the tumor contained the tissue from 3 germ lines, including gut-like epithelial tissues (endoderm), muscle (mesoderm), and epidermis tissues (ectoderm). iPSC, induced pluripotent stem cells; MiPSC, hAMSC-derived iPSCs; hESCs, human embryonic stem cells.