Fig. 2.

DOI-induced head twitching in the same 11 rats (Fig. 1) under the same five conditions: 1) control conditions (i.e., during free access to food and prior to food restriction; circles); 2) during food restriction (10 g/day; triangles); 3) after resumption of free access to food (inverted triangles); 4) after receiving 50 mg/kg of streptozotocin (squares); and 5) after insulin replacement (diamonds). Ordinate: average number of twitches in a 20-min observation period ± SEM. Abscissae: dose in mg/kg body weight. “V” indicates vehicle. *, p<0.05, **, p<0.01, ***, p<0.001, all compared with vehicle control.