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eso1-W804G showed negative genetic interaction with rdp1Δ, raf2Δ, and swi6Δ. (A) Illustration depicting the role of various proteins in heterochromatin formation. (B) The eso1-W804G mutant alone grew normally at 32° and was only slightly sick at 37°. However, combining the eso1-W804G mutation with rdp1, raf2, or swi6 deletion made the cells grow poorly at 37°.
