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END3 mutants display RAS2-dependent ROS accumulation during stationary phase. ROS accumulation in wild-type, end3Δ, and end3Δ ras2Δ stationary phase cells was assayed using H2DCF-DA by flow cytometry (see Materials and Methods). The data from one of three independent assays are displayed on histograms and divided into M1 (low ROS) and M2 (high ROS) populations.
