Table 1. Yeast strains used in this study.
| Strain | Relevant Genotype | Construction Details (Reference) |
|---|---|---|
| EAS28 | Wild-type | MATa his7-2 trp1-289 ura3-52 (Sia et al. 2001) |
| DTK260 | leu2::HisG | EAS28 with pNKY85 (Kelly et al. 2007) |
| DTK264 | ade2-min3 | DTK260 with pDTK123 (Kelly et al. 2007) |
| DTK271 | ade2-min3, MATα | DTK264 with pGal-HO (Herskowitz and Jensen 1991) |
| DTK284 | ade2-min3, arg8::HisG | DTK264 with pDS27 |
| DTK904 | ade2-min3, zrt1::LEU2 | DTK284 with zrt1::LEU2a (Kelly et al. 2007) |
| DTK1012 | ade2-min3, zrt1::LEU2, rad27::KAN | DTK904 with rad27::KANa |
| DTK1056 | ade2-min3, rad50::KAN | DTK271 with rad50::KAN (Kelly et al. 2007) |
| DTK1074 | ade2-min3, rad51::KAN | DTK271 with rad51::KANa |
| DTK1088 | ade2-min3, end3::KAN | DTK271 with end3::KANa |
| DTK1091 | ade2-min3, irc10::KAN | DTK271 with irc10::KANa |
| DTK1174 | ade2-min3, zrt1::LEU, end3::KAN | DTK904 x DTK1088, isolated spore |
| DTK1185 | ade2-min3, end3-1, ras2::KAN | Y797 with ras2::KANa |
| DTK1186 | ade2-min3, ras2::LEU2 | DTK271 with ras2::LEU2a |
| DTK1187 | ade2-min3, end3::KAN, ras2::KAN | DTK1088 x DTK1186, isolated spore |
| DTK1188 | ade2-h7.5 | DTK260 with pKK055, FOAR isolate |
| DNY101 | rad52::URA3 | (Nag and Petes 1993) |
| DTK1199 | ade2-min3, rad27::KAN | DTK271 x DTK1012, isolated spore |
| DTK1205 | jnm1::KAN | Spore isolated from Yeast Deletion Consortium strain dissection |
| DTK1218 | ade2-min3, end3-1, rad27::KAN | Y797 x DTK1199, isolated spore |
| DTK1224 | ade2-min3, rad27::KAN, rad52::URA3 | DTK1199 x DTK1253, isolated spore |
| DTK1225 | ade2-h7.5, rad27::KAN | DTK1188 with rad27::KANa |
| DTK1227 | ade2-min3, end3-1, rad52::URA3 | Y797 x DTK1191, isolated spore |
| DTK1247 | ade2-min3, jnm1::KAN | DTK271 x DTK1205, isolated spore |
| DTK1253 | ade2-min3, rad52::URA3 | DTK1191 x DTK284, isolated spore |
| DTK1266 | ade2-h7.5, end3::KAN | DTK1188 with end3::KANa |
| YKH27 | pkc1-4 | (Huang and Symington 1995) |
| DTK1279 | ade2-min3, pkc1-4 | DTK271 x YKH27, isolated spore |
| DTK1288 | ade2-min3, zrt1::LEU2, pkc1-4 | DTK904 x DTK1279, isolated spore |
| DTK1289 | ade2-min3, rad50::KAN, rad52::URA3 | DTK1268 x DTK284, isolated spore |
| DTK1290 | ade2-min3, rad51::KAN, rad52::URA3 | DTK1269 x DTK284, isolated spore |
| DTK1293 | ade2-min3, end3::KAN, pkc1-4 | DTK1088 x DTK1279, isolated spore |
| DTK1294 | ade2-min3, rad27::KAN, pkc1-4 | DTK1199 x DTK1279, isolated spore |
| DTK1316 | ade2-min3, dnl4::KAN | DTK271 with dnl4::KANa |
| DTK1346 | ade2-min3, pkc1-4, rad52::URA3 | DTK1191 x DTK1279, isolated spore |
| DTK1357 | ade2-min3, pkc1-4, rad50::KAN, rad52::URA3 | DTK1056 x DTK1346, isolated spore |
| DTK1358 | ade2-min3, pkc1-4, rad51::KAN, rad52::URA3 | DTK1074 x DTK1346, isolated spore |
| DTK1360 | ade2-min3, etr1::KAN | DTK271 with etr1::KANa |
| DTK1361 | ade2-min3, por1::KAN | DTK271 with por1::KANa |
| DTK1362 | ade2-min3, pkc1-4, rad50::KAN | DTK271 x DTK1357, isolated spore |
| DTK1363 | ade2-min3, pkc1-4, rad51::KAN | DTK271 x DTK1358, isolated spore |
| DTK1364 | ade2-min3, rad27::KAN, etr1::KAN | DTK1199 x DTK1360, isolated spore |
| DTK1367 | ade2-min3, pkc1-4, etr1::KAN | DTK1279 x DTK1360, isolated spore |
| DTK1368 | ade2-min3, pkc1-4, por1::KAN | DTK1279 x DTK1361, isolated spore |
| DTK1370 | ade2-min3, rad27::KAN, por1::KAN | DTK1199 x DTK1361, isolated spore |
| DTK1371 | ade2-min3, end3::KAN, etr1::KAN | DTK1088 x DTK1360, isolated spore |
| DTK1372 | ade2-min3, end3::KAN, por1::KAN | DTK1088 x DTK1361, isolated spore |
| DTK1373 | ade2-h7.5, end3::KAN | DTK1188 with end3::KANa |
| DTK1375 | ade2-h7.5, pkc1-4 | DTK1188 x DTK1279, isolated spore |
| DTK1379 | ade2-min3, zrt1::LEU, ptc1::KAN | DTK904 with ptc1::KANa |
| DTK1386 | ade2-min3, pkc1-4, ptc1::KAN | DTK1279 x DTK1379, isolated spore |
| DTK1408 | ade2-min3, pkc1-4, dnl4::KAN | DTK1279 x DTK1316, isolated spore |
a
Strain was made using a PCR-generated construct.