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. 2012 Oct 1;2(10):1223–1232. doi: 10.1534/g3.112.003558

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Copyright © 2012 Chang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The esa1 temperature-sensitive growth defect is partially suppressed by four genes expressed from 2μ plasmids. Increased gene dosage of LYS20 (pLP1405), VAP1 (pLP1406), LEU2 (pLP1417), or NAB3 (pLP1419) from a 2μ plasmid moderately suppresses the esa1-414 (LPY3291) growth defect at 35°. The esa1 growth defect at 35°, demonstrated by vector (pLP1402) transformants, is completely restored in cells transformed with an ESA1 plasmid (pLP796). All strains were plated on SC-Ura-Trp media. Suppression was not observed at higher temperatures. Multiple independent transformants were tested to examine any variability between transformants.