Table 2. Plasmids used in this study.
| Plasmid (Alias) | Description | Source/Reference |
|---|---|---|
| pLP362 (pRS426) | Vector URA3 2µ | Sikorski and Hieter 1989 |
| pLP1402 (pRS202) | Library vector URA3 2μ | P. Hieter |
| pLP37 | SIR2 URA3 2μ | |
| pLP271 | Vector TRP1 2µ | |
| pLP796 | ESA1 URA3 2μ | Clarke et al. 2006 |
| pLP798 | ESA1 TRP1 2µ | |
| pLP863 | esa1-414 TRP1 CEN | Clarke et al. 1999 |
| pLP1238 | NAB3 URA3 2μ | |
| pLP1259 | VAP1 URA3 2μ | |
| pLP1310 | NAB3 TRP1 2µ | |
| pLP1412 | LYS20 URA3 2μ | |
| pLP1405 | LYS20-library clone URA3 2μ | |
| pLP1406 | VAP1-library clone URA3 2μ | |
| pLP1417 | LEU2-library clone URA3 2μ | |
| pLP1419 | NAB3-library clone URA3 2μ | |
| pLP2018 | NAB3 URA3 2µ | |
| pLP2054 | NRD1 URA3 2µ |
Except where noted, plasmids were constructed during the course of this study or are part of the standard lab collection. “Library clone” represents a clone obtained directly in the suppressor screen, whereas others are subclones as detailed in Clarke (2001).