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Multiplex nested RT-PCR targetingPfs25andPvs25. Representative 2% agarose gel electrophoresis showing specific amplifications generated from secondary PCR. Lane M, 50-bp ladder marker; lanes 1–6, P. falciparum genomic DNA, P. vivax genomic DNA, water as negative control, P. falciparum cDNA, P. vivax cDNA and mixed P. falciparum and P. vivax cDNA, respectively. Molecular size in base pairs is shown on the left and right of the gel.
