Table 1.
PCR primers used for amplification ofPfs25andPvs25
| Gene target | Primers | Sequences (5′ → 3′) | Nucleotide positions | Product size (bp) |
|---|---|---|---|---|
|
Primary PCR |
|
|
|
|
|
Pfs25 and Pvs25 |
FV25F0 |
GAAGATACATGTGAAGAAAAA |
237–257* or 163–183# |
264 for P. falciparum |
| |
FV25R0 |
ATTGGGAACTTTGCCAATA |
482–500* or 414–432# |
270 for P. vivax |
|
Secondary PCR |
|
|
|
|
|
Pfs25 |
F25F1 |
AAATGTGACGAAAAGACTG |
264–281* |
201 |
| |
F25R1 |
AGTTTTAACAGGATTGCTTGTATC |
441–464* |
|
|
Pvs25 |
V25F1 |
ACCCTAGGCAAAGCATG |
202–218# |
115 |
| V25R1 | CAAGTGTCTTCCTTCAAAGT | 298–317# |