Table 2.
Detection ofPfs25andPvs25in artificial mixed DNA templates by single nested RT-PCRs and multiplex nested RT-PCRs
| DNA templates (no. of copy) | Single nested RT-PCR | Multiplex nested RT-PCR | ||
|---|---|---|---|---|
| |
Pfs25 |
Pvs25 |
Pfs25 |
Pvs25 |
| Pfs25L (0.1) + Pvs25L (106) |
− |
+ |
− |
+ |
| Pfs25L (1) + Pvs25L (106) |
− |
+ |
− |
+ |
| Pfs25L (10) + Pvs25L (106) |
+ |
+ |
+ |
+ |
| Pfs25L (102) + Pvs25L (106) |
+ |
+ |
+ |
+ |
| Pfs25L (105) + Pvs25L (106) |
+ |
+ |
+ |
+ |
| Pfs25L (106) + Pvs25L (105) |
+ |
+ |
+ |
+ |
| Pfs25L (106) + Pvs25L (102) |
+ |
+ |
+ |
+ |
| Pfs25L (106) + Pvs25L (10) |
+ |
+ |
+ |
+ |
| Pfs25L (106) + Pvs25L (1) |
+ |
− |
+ |
− |
| Pfs25L (106) + Pvs25L (0.1) | + | − | + | − |