Table 3.
Microscopy and molecular diagnosis of malaria species and gametocytes in 235 clinical isolates
| Infection | Microscopy | 18S rRNA PCR | Single nested RT-PCR | Multiplex nested RT-PCR | ||
|---|---|---|---|---|---|---|
| |
|
|
Pfs25 |
Pvs25 |
Pfs25 |
Pvs25 |
|
Monoinfection |
|
|
|
|
|
|
|
P. falciparum |
76 |
75 |
− |
− |
− |
− |
| Gametocyte |
8 |
− |
68 |
− |
67 |
− |
|
P. vivax |
80 |
78 |
− |
− |
− |
− |
| Gametocyte |
30 |
− |
− |
74 |
− |
74 |
|
Co-infections |
|
|
|
|
|
|
|
P. falciparum and P. vivax |
1 |
10 |
− |
− |
− |
− |
| Gametocyte |
0 |
− |
9 |
6 |
8 |
6 |
|
P. vivax and P. malariae |
0 |
1 |
− |
− |
− |
− |
| Gametocyte |
0 |
− |
0 |
1 |
0 |
1 |
|
P. falciparum, P. vivax |
0 |
1 |
− |
− |
− |
− |
| and P. malariae |
|
|
|
|
|
|
| Gametocyte |
0 |
− |
0 |
1 |
0 |
1 |
| Total malaria positive cases |
157 |
165 |
− |
− |
− |
− |
| Total P. falciparum |
77 |
86 |
− |
− |
− |
− |
| Gametocyte (%)* |
8 (8.9) |
− |
77 (89.5) |
− |
75 (87.2) |
− |
| Total P. vivax |
81 |
90 |
− |
− |
− |
− |
| Gametocyte (%)** | 30 (31.1) | − | − | 82 (91.1) | − | 82 (91.1) |
* Percentage of P. falciparum gametocyte positives per total P. falciparum positives by 18S rRNA PCR.
** Percentage of P. vivax gametocyte positives per total P. vivax positives by 18S rRNA PCR.
Dashes indicate no applicable data.