Table 4.
Diagnostic performance of multiplex nested RT-PCR forPfs25andPvs25with single nested RT-PCR as reference
| Gametocytes (%) | ||
|---|---|---|
| |
P. falciparum |
P. vivax |
| Sensitivity |
75/77 (97.4) |
81/82 (98.9) |
| Specificity |
80/80 (100) |
80/81 (98.8) |
| Likelihood ratio of positive test |
* |
82.4 |
| Likelihood ratio of negative test |
0.03 |
0.01 |
| Ratio of single nested RT-PCR positives to |
9.63 |
2.73 |
| microscopy positives |
|
|
| Ratio of multiplex nested RT-PCR positives to |
9.50 |
2.73 |
| microscopy positives |
* Denominator is zero.