Figure 3.

Protoplast isolation rates of American elm callus grown in various concentrations of AIP. American elm callus cells were grown on MSO basal medium supplemented with 5 μM BA, 1 μM NAA, and various levels of AIP and the percentage that developed into protoplasts after a 4 h incubation in a cell wall degrading enzyme mixture were counted. a; American elm callus grown without AIP after digestion, b; American elm callus grown on 100 μM AIP after digestion, c; Protoplast conversion rates for American elm callus grown on varying levels of AIP. Bars with the same letters were not significantly different based on a means separation with Tukey’s adjustment with a p-value of 0.05.