Figure 4. Identifying the docking segments for WASP WCA, ciboulot domain 1, and Tβ4 in their binding to G-actin, by finding the fragments with the highest rate constants for the docking step.

The sequences of the three proteins are aligned. Regions forming helices are indicated by cylinders. In all three cases, the proposed docking segments are the fragments ending at the third position of the conserved “LKK” motif (ending residues shown in red). The corresponding rate constants for the docking step are indicated by a vertical dashed line. Inclusion of the C-terminal residues in italic in the proposed docking segments produced large gaps in N _c, indicating that these residues must belong to the coalescing segments instead.