| Strains |
|
|
| RN4220 |
Laboratory strain; rsbU-
|
[44]
|
| WCUH29 |
Clinical human isolate; rsbU
+
|
[45]
|
| JRN0105 |
RN4220::Pspac-gcp, spac promoter regulated gcp expression mutant |
[3]
|
| JRN0110 |
JRN0105 with plasmid plux- Pilv
|
This study |
| JW290111 |
WCUH29 Δgcp attB:: Pspac-gcp containing plasmid pYH4-lacI |
This study |
| JW290211 |
WCUH29 Δilv-leu containing plasmid pYH4-lacI |
This study |
| JW290311 |
WCUH29 Δilv-leu Δgcp attB:: Pspac-gcp containing plasmid pYH4-lacI |
This study |
| WCUH29/gcp-as |
WCUH29 containing TetR regulated gcp antisense expression plasmid pYH4/gcp-as |
[4]
|
| WCUH29/eno-as |
WCUH29 containing TetR regulated eno antisense expression plasmid pYH4/eno-as |
[24]
|
| Plasmids |
|
|
| plux-Pilv
|
ilv promoter fused with luxABCDE on pFF40 [38]
|
This study |
| pYH4-lacI |
lacI gene inserted in the vector pYH4 at EcoR I site |
This study |
| pLH1 |
The integration vector pLL39 [39] carrying Pspac-lacI segment [46]
|
This study |
| pLH1/gcp |
pLH1 vector carrying gcp gene under regulation of Pspac promoter |
This study |
| pYH4/gcp-as |
pYH4 vector carrying a gcp fragment in an antisense orientation |
[3]
|
| pKOR1 |
E.coli/ S.aureus shuttle vector, permits lambda recombination and ccdB selection;temperature sensitive in S. aureus, |
[41]
|
| pKOR1/Δgcp |
pKOR1 vector carrying gcp flanking sequences for gcp allelic replacement |
This study |
| pKOR1/Δilv-leu |
pKOR1 vector carrying operon ilv-leu flanking sequences for operon ilv-leu allelicreplacement |
This study |
