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. 2012 Oct 4;7(10):e45694. doi: 10.1371/journal.pone.0045694

Figure 4. The mRNA of Adfp and Tip47 level was compared using quantitative RT-PCR.

Figure 4

Adfp expression increased 12 hours and 48 hours after heat treatment (A). The protein expression level of Adfp and Tip47 was measured by Western blotting. Adfp is increased in the mouse testes 48 hours after heat treatment (B). Indirect immunofluorescence of Adfp (C) and Tip47 (D) reveals increasing intensity of Adfp 48 hours after heat treatment, especially in the seminiferous tubules associated with large numbers of degenerated and apoptotic germ cells (white arrow). **P<0.001, Scale bar = 100 µm.