Figure 5. The trapping cassette of Adfp ^Δ1/Δ1 mice is inserted in the intron between exons 1 and 2, before the Adfp translation start site.

The peroxisome proliferator-activated receptor responsive element (PPRE) is located ∼2,000 bp upstream of the transcription start site (A). RT-PCR products are presented using primers targeted to exons 1–3, exons 3–8, and exons 6–8 of the Adfp transcripts of testes and liver from Adfp ^Δ1/Δ1 mice and wild type mice. β-actin was amplified as the control gene (B).