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. 2012 Oct 4;7(10):e46865. doi: 10.1371/journal.pone.0046865

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© 2012 Stotz, Clapham

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–D) % Block of S2R+ I_Clswell, I_dBest1, HEK I_Clswell and I_d64m by 1 mM furosemide, 100 µM DIDS, and 30 µM DCPIB. Block at 0 mV is presented to emphasize the incomplete voltage-dependent DCPIB block of S2R+ I_Clswell and I_dBest1. (A) Steady state S2R+ I_Clswell activated by 200 mOSM stimulation was blocked 96% ±1.6 by furosemide, 19% ±4 by DIDS, and 52% ±10.6 by DCPIB. * no difference compared to I_dBest1 block and significantly different compared to HEK I_Clswell and I_d64m (ANOVA, p<0.05). (B) I_dBest1, stimulated for 2 min by 200 mOSM, was blocked 96% ±1.7 by furosemide, 44% ±10 by DIDS, and 47% ±10.9 by DCPIB. * no difference compared to S2R+ I_Clswell block and significantly different compared to HEK I_Clswell and I_d64m (ANOVA, p<0.05). (C) Steady-state HEK I_Clswell activated by 200 mOSM stimulation was blocked 7% ±3.5 by furosemide, 77% ±3 by DIDS, and 99% ±0.7 by DCPIB. (D) Constitutive I_d64m (320 mOSM) was blocked 77% ±3 by furosemide, 98% ±1.5 by DIDS, and 98% ±1.2 by DCPIB. significantly different compared to S2R+ I_Clswell, HEK I_Clswell and I_d64m (ANOVA, p<0.05). (E–F) I–V relations for S2R+ I_Clswell and I_dBest1 demonstrate DCPIB voltage-dependent block. At 80 mV, DCPIB block of S2R+ I_Clswell is 90% ±3.6 (n = 6), and 82% ±6.5 for I_dBest1 (n = 7).

Inline graphic — (A–D) % Block of S2R+ I_Clswell, I_dBest1, HEK I_Clswell and I_d64m by 1 mM furosemide, 100 µM DIDS, and 30 µM DCPIB. Block at 0 mV is presented to emphasize the incomplete voltage-dependent DCPIB block of S2R+ I_Clswell and I_dBest1. (A) Steady state S2R+ I_Clswell activated by 200 mOSM stimulation was blocked 96% ±1.6 by furosemide, 19% ±4 by DIDS, and 52% ±10.6 by DCPIB. * no difference compared to I_dBest1 block and significantly different compared to HEK I_Clswell and I_d64m (ANOVA, p<0.05). (B) I_dBest1, stimulated for 2 min by 200 mOSM, was blocked 96% ±1.7 by furosemide, 44% ±10 by DIDS, and 47% ±10.9 by DCPIB. * no difference compared to S2R+ I_Clswell block and significantly different compared to HEK I_Clswell and I_d64m (ANOVA, p<0.05). (C) Steady-state HEK I_Clswell activated by 200 mOSM stimulation was blocked 7% ±3.5 by furosemide, 77% ±3 by DIDS, and 99% ±0.7 by DCPIB. (D) Constitutive I_d64m (320 mOSM) was blocked 77% ±3 by furosemide, 98% ±1.5 by DIDS, and 98% ±1.2 by DCPIB. significantly different compared to S2R+ I_Clswell, HEK I_Clswell and I_d64m (ANOVA, p<0.05). (E–F) I–V relations for S2R+ I_Clswell and I_dBest1 demonstrate DCPIB voltage-dependent block. At 80 mV, DCPIB block of S2R+ I_Clswell is 90% ±3.6 (n = 6), and 82% ±6.5 for I_dBest1 (n = 7).