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. 2012 Oct 4;7(10):e46958. doi: 10.1371/journal.pone.0046958

Figure 5. Effect of the silane AB110873 on the activation of mineralocorticoid (MR) and glucocorticoid receptors (GR).

Figure 5

The impact of silane AB110873 on MR and GR transactivation was measured in HEK-293 cells transfected with MMTV-LacZ reporter, MR (A,B) or GR (C) and luciferase transfection control. Cells were incubated with the receptor ligands for 24 h, followed by analysis of reporter activity as given in the Methods section. A, Concentration-dependent activation of the human MR by the silane AB110873. Maximal activation at 30 µM was set as 100%. B, effect of the antagonist spironolactone (1 µM) on MR activation by aldosterone (5 nM) or by the silane AB110873 (20 µM); C, activation of human GR by cortisol (100 nM) and effect of AB110873 (20 µM) and antagonist RU-486 (1 µM) (MR and GR activity of the vehicle control was set as 1, data represent fold activation). Data were obtained from four independent experiments, each performed in triplicates (mean ± SD).