Table 1.
Primers used to make deletion constructs.
| Primer name | Sequence (5′-3′) | Plasmid |
|---|---|---|
| CS11fow | GCTTACTTTCATAATTGCTTTTGATTTTAACGACTTTTAACG | pCSΔ94-73 |
| CS11rev | CGTTAAAAGTCGTTAAAATCAAAAGCAATTATGAAAGTAAGC | |
| CS12fow | GCTTACTTTCATAATTGCCGACAACTTGAGAAGATCAAAAAAC | pCSΔ94-51 |
| CS12rev | GTTTTTTGATCTTCTCAAGTTGTCGGCAATTATGAAAGTAAGC | |
| CS13fow | GCTTACTTTCATAATTGCAAAAAACAACTAATTATTCGAAACGAGG | pCSΔ94-33 |
| CS13rev | CCTCGTTTCGAATAATTAGTTGTTTTTTGCAATTATGAAAGTAAGC | |
| CS14fow | GCTTACTTTCATAATTGCCGAAACGAGGAATTCATGCCAGGGG | pCSΔ94-16 |
| CS14rev | CCCCTGGCATGAATTCCTCGTTTCGGCAATTATGAAAGTAAGC | |
| CS15fow | CTTTTTTTTTATCATCATTATTAGCCGAAACGAGGAATTCATGCCAGGGG | pCSΔ110-16 |
| CS15rev | CCCCTGGCATGAATTCCTCGTTTCGGCTAATAATGATGATAAAAAAAAAG | |
| CS25fow | GCTTACTTTCATAATTGCGATTTTAACGACTTTTAACGAC | pCSΔ94-69 |
| CS25rev | GTCGTTAAAAGTCGTTAAAATCGCAATTATGAAAGTAAGC | |
| CS30fow | GCTTACTTTCATAATTGCTAACGACTTTTAACGAC | pCSΔ94-64 |
| CS30rev | GTCGTTAAAAGTCGTTAGCAATTATGAAAGTAAGC | |
| AMYAOX5UTRdel5TOP | GGTTCCAATTGACAAGCATTTTAACGAC | pCSΔ69-73 |
| AMYAOX5UTRdel5BOTTOM | GTCGTTAAAATGCTTGTCAATTGGAACC | |
| AMYAOX5UTRdel9TOP | GCGACTGGTTCCAATTGACAATTTAACGACTTTTAACG | pCSΔ67-75 |
| AMYAOX5UTRdel9BOTTOM | CGTTAAAAGTCGTTAAATTGTCAATTGGAACCAGTCGC | |
| PAH15top | CTAGTAGCTTACTTTCATAATTGCACA | pCSΔ94-80 |
| PAH15bottom | AGCTTGTGCAATTATGAAAGTAAGCTA | |
| TM31-43TOP | GACAAGCTTTTGATTTCGACAACTTGAGAAG | pCSΔ63-51 |
| TM31-43BOT | CTTCTCAAGTTGTCGAAATCAAAAGCTTGTC | |
| MN62-79TOP | CAACTTGAGAAGATCCGAAACGAGGAATTC | pCSΔ32-16 |
| MN62-79BOT | GAATTCCTCGTTTCGGATCTTCTCAAGTTG | |
| KO74-86TOP | GATCAAAAAACAACTAATAGGAATTCATGCCAG | pCSΔ9-19 |
| KO74-86BOT | CTGGCATGAATTCCTATTAGTTGTTTTTTGATC | |
| MNKOTM25-28DELBOT | CGTTAAAAGTCGTTAAAAAAGCTTGTCAATTGG | pCSΔ67-70 |
| MNKOTM25-28DELTOP | CCAATTGACAAGCTTTTTTAACGACTTTTAACG | |
| MNKOTM66-73DELTOP | CTTGAGAAGATCAAAAATTATTCGAAACGAGG | pCSΔ22-29 |
| MNKOTM66-73DELBOT | CCTCGTTTCGAATAATTTTTGATCTTCTCAAG |