Fig. 6.

Effect of Ara-C and the AurA inhibitor on NB4 and KG1 cells in relation to p38 MAP kinase activation and NF-κB protein. (A) Cells were treated with various concentrations (0.01, 0.1, 1, 5, and 10 µM) of Ara-C for 48 h, after which apoptosis was evaluated by flow cytometry. (B) Expression of p38 and phospho-p38 was evaluated after Ara-C (2 µM) treatment. Cells were treated with a selective inhibitor of p38, SB203580 (20 µM), for 3 h; next, the rates of apoptosis were compared. (C) NB4 and KG1 cells were treated with various concentrations of Ara-C or the AurA inhibitor (0.01, 0.1, 1, 5, and 10 µM) and then evaluated for apoptosis by flow cytometry. (D) Expression of NF-κB evaluated by western blot analysis.