Fig. 2.

(a) IF findings with urine precipitates after 453,000 g centrifugation of urine from a diabetic patient with normoalbuminuria. The anti-PCX monoclonal antibody 22A4 showed fine granular structures. Original magnification ×400. (b) IEM findings for the urine sample from a normoalbuminuric diabetic patient. Scale bar 100 nm. (c) The presence of PCX was confirmed by western blot analysis. The urine precipitate after centrifugation at 453,000 g of urine from two patients (patient 1 was a normoalbuminuric patient and patient 2 had IgA nephropathy) and glomerular lysate were used as samples with two anti-PCX monoclonal antibodies (22A4 and #5). Gl, glomerular lysate; M, molecular size marker; P1, patient 1; P2, patient 2