Figure 4.

The Germline Nuclear RNAi/Chromatin Pathway Acts in trans but Cannot Exit the Germline

(A and B) trans-heterozygous animals were generated by crossing SX1866 hermaphrodites with piRNA sensor males. Strain SX1866 expressing H2B-GFP under control of the ubiquitous dpy-30 promoter was generated by MosSCI into ttTi5606 on chromosome II (mjSi1[dpy-30::his-58::gfp::tbb-2]). DIC and fluorescence microscopy of animals from the parental line (A) or of trans-heterozygous animals from the cross (B). Note that the parental line expresses H2B-GFP from two copies in the genome and is therefore brighter. Yellow arrowheads indicate germ cell nuclei; red arrowheads indicate somatic (intestinal) cell nuclei.