Table 3.

Optimization of PNA Delivery

PNA designation and sequence
Anti-acpP peptide-PNA		MIC (μM)*Pseudomonas aeruginosa (PA01)
3934	H-KKH RKH RKH RKH-Ahx-ctc ata ccttg-NH2	>5
3966	H-(KFF KFF KFF K)D-Ahx-ctc ata ccttg-NH2	5
3930	H-FWR IRIR R-Ahx-ctc ata ccttg-NH2	>5
3931	H-FWR RFW RR-Ahx-ctc ata ctt tg-NH2	>5
3968	H-(RFR)4-Ahx-(βa1a)-ctc ata ctt tg-NH2	3
3969	H-(R-Ahx)6-(βala)-ctc ata cct tg-NH2	2
3984	H-(R-Ahx-R)4-Ahx-(βa1a)-ctc ata cct tg-NH2	.8
Mismatch peptide-PNA
3935	H-KKH RKH RKH RKH-Ahx-ctc cta cat tg-NH2	>5
3967	H-(KFF KFF KFF K)D-Ahx-ctc cta cat tg-NH2	5
3932	H-FWR IRIR R-Ahx-ctc cta cat tg-NH2	>5
3933	H-FWR RFW RR-Ahx-ctc cta cat tg-NH2	>5
3992	H-(RFR)4-Ahx-(βa1a)-ctc cta cat tg-NH2	3
3993	H-(R-Ahx)6-(βala)-ctc cta cat tg-NH2	6
3985	H-(R-Ahx-R)4-Ahx-(βa1a)-ctc cta cat tg-NH2	2

^*MIC values are reported as the concentration resulting in an OD value <0.15 after 8 hours incubation at 37°C.

Ahx, 6-aminohexanoic acid; βala: β-alanine.