Table 4.

Antimicrobial Activity of Antisense Conjugates

		MIC (μM)*Pseudomonas aeruginosa
		PA01	PA14	LESB58
Anti-acpP peptide-PNA
3969	H-(R-Ahx)6-(βala)-ctc ata cct tg-NH2	2	2	2
3984	H-(R-Ahx-R)4-Ahx-(βala)-ctc ata cct tg-NH2	2	2	2
Mismatch peptide-PNA
3993	H-(R-Ahx)6-(βala)-ctc cta cat tg-NH2	20	>10	10
3985	H-(R-Ahx-R)4-Ahx-(βala)-ctc cta cat tg-NH2	5	>4	>2
Anti-ftsZ peptide-PNA
4071	H-(R-Ahx)6-(βala)-cca gtt caa a-NH2	2	2	1.5
4073	H-(R-Ahx-R)4-Ahx-(βala)-cca gtt caa a-NH2	1	2	.6
Mismatch peptide-PNA
4070	H-(R-Ahx)6-(βala)-cta gtc caa a-NH2	12	>10	>1.5
4072	H-(R-Ahx-R)4-Ahx-(βala)-cta gtc caa a-NH2	5	>4	.6
Antibiotic Controls
Gentamicin		.3	.3	1
Ciprofloxacin		.3	.3	2
Ofloxacin		2	2	>6
Ceftazidime		2	3	>10

^*MIC values are reported as the concentration resulting in an OD value <0.15 after 20 hours incubation at 37°C (PA14 growth inhibition data are shown in Figure S1).

Ahx, 6-aminohexanoic acid; βala, β-alanine.