Figure 3. Adult Tamoxifen exposure in PlpCre;Nf1fl/fl mice causes neurofibroma formation.
(A) Kaplan-Meyer survival curve (p < 0.0001). (B) Gross dissection of cervical spinal cord with GEM Grade I neurofibromas in a PlpCre+;Nf1fl/fl animal. Ruler shows 1mm markings. (C) Tissue sections of a GEM Grade I neurofibroma in a PlpCre+;Nf1fl/fl animal after adult tamoxifen. Upper panels are magnified 10×; lower panels are magnified 25×. Red arrows highlight mast cells. (D) PCR analysis shows the Nf1 floxed allele (NF flox) and the recombined Cre allele (R-Cre). Each lane contains 1ug of DNA. (O.N. = optic nerve; C.C. = corpus callosum; S.N. = sciatic nerve; DRG = dorsal root ganglia; B.M. = bone marrow).