Table 1.

VSD activation in response to laminar specific photostimulation, and interlaminar functional projection strength in normal ACSF.

(A) LAYER-SPECIFIC VSD ACTIVATION IN PHOTOSTIMULATED AND OUTPUT LAYERSa
Photostimulated cortical layer	Laminar specific activated pixels				Total activated pixels across layers 2/3–6
	Layer 2/3	Layer 4	Layer 5	Layer 6
Layer 2/3	61.17 ± 10.92 (mean ± SE)	20.32 ± 6.77	68.67 ± 23.91	17.77 ± 6.7	167.92 ± 26.58
Layer 4	187.91 ± 44.7	175.59 ± 31.76	150.73 ± 30.14	53.14 ± 9.98	569.01 ± 93.85
Layer 5	35.05 ± 15.51	19.31 ± 5.38	90.44 ± 12.45	38.79 ± 8.01	221.76 ± 64.75
Layer 6	2.14 ± 0.42	2.18 ± 0.4	10.25 ± 4.08	83.4 ± 14.95	97.57 ± 17.87
(B) QUANTITATIVE DATA OF INTERLAMINAR FUNCTIONAL PROJECTION STRENGTHb
Photostimulated cortical layer	Interlaminar projection strength (Qproj)
	Layer 2/3	Layer 4		Layer 5	Layer 6
Layer 2/3		0.29 ± 0.10 (mean ± SE)		0.48 ± 0.16	0.19 ± 0.50
Layer 4	1.38 ± 0.33			0.86 ± 0.13	0.31 ± 0.18
Layer 5	0.76 ± 0.3	0.45 ± 0.15			0.47 ± 0.11
Layer 6	0.02 ± 0.0	0.05 ± 0.01		0.12 ± 0.02

^aPhotostimulation-evoked VSD activation was quantified by the average number of activated pixels in the image frame at the defined peak response phase. Data quantification for each laminar location was based upon 7–11 VSD image datasets.

^bSee the section “Materials and Methods” for quantification of interlaminar functional projection strength (Q_proj).