Figure 3.

Formation of the OBP-oriS* complex depends on complementary intrastrand base pairing between box III and box I. (A) The palindrome consisting of box III and box I is shown. The recognition sequences for OBP, GTTCGCAC, are highlighted in bold. Mutations are indicated by boxes. (B) Autoradiograph of a gel retardation experiment. OriS* was produced by heat treatment of radiolabeled SphI-EcoRI restriction fragments from pORI(wt), pORI(mut8), pORI(mut9), pORI(mut10), and pORI(mut11). The formation of complexes between OBP and oriS* was examined by agarose gel electrophoresis.