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. 2001 Jun 19;98(13):7194–7199. doi: 10.1073/pnas.121177198

Figure 4.

Figure 4

Single-stranded oligonucleotides corresponding to oriS* are efficient activators of ATP hydrolysis. (A) ATP hydrolysis by OBP. The DNA cofactors are single-stranded oligonucleotides that can adopt an oriS* conformation as described in Fig. 2A. AwtBwt, AmutBwt, AwtBmut, AmutBmut, and AmutdT29 are 63-mers described in Table 1. OriS80 is an 80-mer corresponding to the upper strand of oriS. The dashed line indicates DNA-independent hydrolysis of ATP. T65 is a 65-mer of oligodeoxythymidylate. (B) Stimulation of ATP hydrolysis depends on the length of the 3′ tail. The DNA cofactors are AwtdT3, AwtdT6, AwtdT8, AwtdT10, AwtdT20, and AwtdT29 (●). The DNA cofactor AmutdT10 (○) has transversions in the A domain as described in Materials and Methods.