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Effects of region swapping on stereoselectivity of (+)-and (−)-pinoresinol-forming dirigent proteins. Chiral HPLC analyses of pinoresinol (2) antipodes are shown. A, recombinant native ScDIR. B, recombinant native AtDIR6. C, AtDIR6 Region-A swapped into ScDIR. D, AtDIR6 Region-B swapped into ScDIR.
