TABLE 2.
Top candidate proteins elevated in androgen-independent cell lines based on average normalized spectral count values
Fifty-seven proteins were found with a minimum of two peptides in at least three AIPC cell lines and with less than one peptide in androgen-dependent and normal prostate epithelial cell lines. For a complete list of the 52 proteins, see supplemental Table S10.
| RWPE (normal) | LNCaP (AD)a | VCaP (AD) | 22Rv1 (AI)b | DU145 (AI) | PC3 (AI) | PPC1 (AI) | LNCaP-SF (AI) | Number of positive AIPC cell lines | Previously studied during prostate cancer progression? | |
|---|---|---|---|---|---|---|---|---|---|---|
| PROS1 | 0 | 0 | 0 | 8 | 20 | 3 | 20 | 23 | 5 | |
| TWSG1 | 1 | 0 | 0 | 5 | 6 | 8 | 7 | 9 | 5 | |
| GBA | 0 | 1 | 0 | 4 | 25 | 5 | 37 | 7 | 5 | Ref. 28 |
| PGRMC1 | 0 | 1 | 0 | 3 | 3 | 2 | 5 | 4 | 5 | |
| LTBP1 | 0 | 0 | 0 | 0 | 11 | 58 | 161 | 18 | 4 | |
| LTBP3 | 0 | 0 | 0 | 1 | 27 | 28 | 48 | 22 | 4 | |
| HTRA1 | 1 | 1 | 0 | 0 | 11 | 10 | 72 | 12 | 4 | |
| MAN1A1 | 0 | 0 | 0 | 0 | 2 | 5 | 93 | 3 | 4 | |
| COL6A2 | 0 | 0 | 0 | 0 | 5 | 38 | 23 | 30 | 4 | |
| MGAT5 | 0 | 0 | 0 | 0 | 14 | 10 | 61 | 3 | 4 | Ref. 26 |
| PAM | 0 | 0 | 0 | 0 | 32 | 8 | 34 | 8 | 4 | Ref. 27 |
| PCSK1N | 0 | 0 | 0 | 30 | 20 | 24 | 0 | 11 | 4 | |
| CHID1 | 0 | 0 | 0 | 0 | 2 | 7 | 50 | 11 | 4 | |
| ROBO1 | 0 | 1 | 0 | 2 | 0 | 9 | 25 | 10 | 4 | Ref. 29 |
| CD59 | 1 | 1 | 0 | 0 | 2 | 9 | 16 | 6 | 4 | Ref. 30 |
| B4GALT4 | 0 | 0 | 0 | 0 | 6 | 6 | 14 | 5 | 4 | |
| B3GAT3 | 0 | 0 | 0 | 3 | 7 | 1 | 10 | 3 | 4 |
a AD, androgen-dependent cell lines.
b AI, androgen-independent cell lines.