FIGURE 4.

Lats2 inhibits the phosphorylation of KIBRA on Ser⁵³⁹ via PP1. A, HEK293T cells were transfected with FLAG-KIBRA and plasmids as indicated. At 48 h post-transfection, the cells were lysed and immunoprecipitated with FLAG antibody. The immunoprecipitates were probed with anti-phospho-Ser⁵³⁹ KIBRA and subsequent anti-FLAG antibodies. B, HeLa cells were transfected with FLAG-KIBRA and control siRNA (lane 1) or siRNA against Lats2 (lane 2) for 48 h. FLAG-KIBRA was immunoprecipitated and probed with the indicated antibodies. Total cell lysates without immunoprecipitation were also analyzed. C, HeLa cells were transfected with control siRNA (lanes 1 and 2) or siRNA targeting PP1 (lane 3) and plasmids as indicated. FLAG-KIBRA was immunoprecipitated and probed with phospho-KIBRA Ser⁵³⁹ and subsequent anti-FLAG antibodies. Total cell lysates without immunoprecipitation were also analyzed. M(K) indicates positions where the relevant molecular markers migrated.