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Phosphoinositide specificity of dAmp-BAR and EndoA1-BAR. Kinetic sensorgrams show that both N-BAR domains have the highest affinity for POPC/POPS/PtdIns(4,5)P2 (77:20:3) and POPC/POPS/PtdIns(3,4,5)P3 (77:20:3) vesicles. Only the association phases of the sensorgrams are shown for clear illustration. All measurements were performed at 23 °C in 20 mm Tris-HCl buffer, pH 7.4 with 0.16 m KCl. Protein concentration was 0.2 μm. RU, resonance units.
