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. 2012 Aug 11;287(41):34078–34090. doi: 10.1074/jbc.M112.372789

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Time course of GUV tubulation by dAmp-BAR and EndoA1-BAR and their respective mutants. A, the time course of the percentage of tubulated POPC/POPS/PtdIns(4,5)P₂/Rh-PE (76.5:20:3:0.5) GUVs caused by EndoA1-BAR WT was measured as a function of protein concentration (1–8 μm). B, the time course of the percentage of tubulated GUVs caused by EndoA1-BAR WT was measured as a function of PtdIns(4,5)P₂ concentration in POPC/POPS/PtdIns(4,5)P₂/Rh-PE (79.5 − x:20:x:0.5) GUVs (x = 0–3 mol %). Protein concentration was 0.5 μm. C, the time courses of the percentage of tubulated POPC/POPS/PtdIns(4,5)P₂/Rh-PE (76.5:20:3:0.5) GUVs caused by dAmp-BAR WT, the H₀ deletion mutant (ΔH₀), and the cationic patch mutant (CPM). Protein concentrations were 0.5 μm. D, the time courses of the percentage of tubulated POPC/POPS/PtdIns(4,5)P₂/Rh-PE (76.5:20:3:0.5) GUVs caused by EndoA1-BAR WT, the H₀ deletion mutant (ΔH₀), the H_i deletion mutant (ΔH_i), and the cationic patch mutant (CPM). Protein concentrations were 0.5 μm. All measurements were performed at 37 °C in 20 mm Tris-HCl buffer, pH 7.4 with 0.16 m KCl.