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. 2012 Aug 17;287(41):34149–34156. doi: 10.1074/jbc.M112.382812

FIGURE 3.

FIGURE 3.

Dectin-1 triggers recruitment of LC3II to phagosomes. A, RAW264.7 cells stably expressing Dectin-1 (DecRAW) or BMDC were pulsed with β-glucan particles (25 μg/ml) and chased for 15 min. Cells were stained for LC3II (green) and F-actin (red) and analyzed by immunofluorescence microscopy. B, BMDC were stimulated with β-glucan particles, chased for the indicated times, and stained as in A. LC3II+ phagosomes per number of cells (n >90) were counted. C, cells were imaged as in A except they were pulsed with 3-μm latex beads. D, cells stably expressing a control vector or an shRNA targeting ATG5 (ATG5 KD) were pulsed for 15 min with β-glucan particles (25 μg/ml). Cells were fixed, permeabilized, and stained for LC3II and F-actin. E, Dectin-1-deficient BMDC were pulsed with β-glucan particles (25 μg/ml) for 15 min. Cells were stained as indicated and analyzed by immunofluorescence microscopy. F, RAW267.4 cells stably expressing a streptavidin-binding peptide epitope-tagged Dectin-1 were stimulated for 15 min with streptavidin-coated beads (100 μg/ml) and imaged as in E.