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Intrinsic fluorescence of hnRNP-U bound to the NEIL1 interacting domain. A, change in the tryptophan fluorescence of hnRNP-U in the presence of increasing concentrations of the NEIL1 C-terminal peptide (residues 312–389). AU, arbitrary units. B, binding isotherm of NEIL1 with hnRNP-U monitored by fluorescence at 338 nm. The calculated affinity (kd) is shown.
