FIGURE 8.

hnRNP-U and NEIL1 protect HEK293 cells from oxidative stress. A, survival of HEK293 cells transfected with control siRNA or siRNA for hnRNP-U, NEIL1, or hnRNP-U + NEIL1. At 48 h after transfection, 400 cells were plated into 60-mm dishes and treated with increasing concentration of GO (1–100 ng/ml medium) for 15 min. After changing to fresh medium, cells were maintained for 10 days before the colonies were stained with crystal violet. Other details are given under “Experimental Procedures.” Error bars indicate S.D. B, Western analysis of hnRNP-U and NEIL1 levels in HEK293 cells 48 h after transfection with corresponding siRNA.