FIGURE 4.
C1orf124 displays preferential binding to replicative and translesion DNA polymerases before and after UV damage, respectively. A, TAP was performed using 293T cells stably expressing SFB-tagged C1orf124 following UV treatment. The results from mass spectrometry analysis are shown. 293T cells were transfected with plasmids encoding SFB-tagged POLD3 (B), SFB-tagged PDIP1 (C), and SFB-tagged POLH (D) together with plasmid encoding Myc-C1orf124. Cells were left untreated or treated with 100 J/m2 UV-C light and collected 4 h later. Coprecipitation was carried out using S-protein beads, and immunoblotting was performed using the indicated antibodies. WB, Western blot.