FIGURE 5.

C1orf124 acts with VCP in promoting cell survival following UV damage. A, C1orf124 interacts with VCP via the SHP box. 293T cells were transfected with plasmids encoding Myc-tagged full-length C1orf124, C1orf124ΔSHP, C1orf124ΔPIP, and C1orf124ΔUBZ together with plasmid encoding SFB-tagged VCP. Coprecipitation was carried out using S-protein beads, and immunoblotting was performed using the indicated antibodies. WB, Western blot. B, interaction of C1orf124 with VCP and PCNA is crucial for the in vivo function of C1orf124. C1orf124-depleted (C1orf124 shRNA1) HeLa cells stably expressing shRNA-resistant (shR) wild-type C1orf124, C1orf124ΔSHP, C1orf124ΔPIP, or C1orf124ΔUBZ were generated. Cells were treated with the indicated doses of UV light and incubated for 14 days. Survival curves are shown for the indicated cell lines. Data are presented as means ± S.D. from three different experiments. C, model depicting the molecular function of C1orf124 in regulating TLS as described under “Discussion.” RPA, replication protein A.